LMP Seminar: Nanopore-powered DNA turbines
LMP Seminar
- Datum: 09.08.2022
- Uhrzeit: 14:00 - 15:30
- Vortragende(r): Dr. Xin Shi
- Dept.of Bionanoscience, Faculty of Applied Sciences, Delft University of Technology, Netherlands
- Ort: Max-Planck-Institut für Dynamik und Selbstorganisation (MPIDS)
- Raum: Video conference at www.zoom.us Meeting ID: 997 1155 2453 Passcode: 771001
- Gastgeber: MPIDS / LMP
- Kontakt: golestanian-office@ds.mpg.de
Our societies have flourished because of macroscale machinery powered by engines and motors. And we are not alone in our reliance on active machines: life itself depends on energy-consuming nanoscale machines, as work at the nanoscale is being done by millions of sophisticated molecular motors. However, until today, designing and building active energy-consuming machines at the nanoscale has remained challenging.
In this talk, I will be presenting our latest results on designing and building nanoscale DNA turbines: DNA nanostructures on nanopores that can autonomously convert transmembrane electrochemical potentials into rotary motion, similar to natural rotary motor proteins such as F0F1-ATP synthase and bacterial flagella motors. We have successfully designed and built two generations of such nanoturbines: a self-organized DNA active rotor (1), and a bottom-up designed chiral-shaped DNA turbine (2). We observed sustained unidirectional rotary motion of these nanoturbines at the single-molecule level as we apply a voltage or salt gradient across the nanopore. These exciting results lay the groundwork, both theoretically and experimentally, for further studies and the development of autonomous nanomachines that leverage autonomous, unidirectional rotational motion.
1. Xin Shi, A-K Pumm, J. Isensee, W. Zhao, D. Verschueren, A. Martin-Gonzalez, R. Golestanian, H. Dietz, C. Dekker, Sustained unidirectional rotation of a self-organized DNA rotor on a nanopore. arXiv:2206.06613. (2022)
2. Xin Shi, A-K Pumm, C M Maffeo, W. Zhao, D. Verschueren, A. Aksimentiev, H. Dietz, C. Dekker. A nanopore-powered DNA nanoturbine. arXiv:2206.06612. (2022)
In this talk, I will be presenting our latest results on designing and building nanoscale DNA turbines: DNA nanostructures on nanopores that can autonomously convert transmembrane electrochemical potentials into rotary motion, similar to natural rotary motor proteins such as F0F1-ATP synthase and bacterial flagella motors. We have successfully designed and built two generations of such nanoturbines: a self-organized DNA active rotor (1), and a bottom-up designed chiral-shaped DNA turbine (2). We observed sustained unidirectional rotary motion of these nanoturbines at the single-molecule level as we apply a voltage or salt gradient across the nanopore. These exciting results lay the groundwork, both theoretically and experimentally, for further studies and the development of autonomous nanomachines that leverage autonomous, unidirectional rotational motion.
1. Xin Shi, A-K Pumm, J. Isensee, W. Zhao, D. Verschueren, A. Martin-Gonzalez, R. Golestanian, H. Dietz, C. Dekker, Sustained unidirectional rotation of a self-organized DNA rotor on a nanopore. arXiv:2206.06613. (2022)
2. Xin Shi, A-K Pumm, C M Maffeo, W. Zhao, D. Verschueren, A. Aksimentiev, H. Dietz, C. Dekker. A nanopore-powered DNA nanoturbine. arXiv:2206.06612. (2022)